Determining the best dose of Ovulin hormone injection in artificial reproduction of Cyprinus carpio by examining some blood factors and steroid hormones

This study was performed to evaluate the best dose of Ovulin hormone in artificial reproduction of female sea carp broodstocks in the spring of 2021 in the bone reproduction and breeding center of Sijual in Bandar-e-Turkmen, Golestan province. 12 adult female sea carp with an average weight of 1750.46 ± 312.58 g were injected at doses of 0.4, 0.5, 0.6 and 0.7 ml / kg body weight of Ovulin hormone. Blood samples were taken from fish in two stages. The first stage was before injection and the second stage was 12-12 hours after injection. The results of hematological indices showed that injection of different doses of Ovulin hormone had no effect on the number of red blood cells, white blood cells, hemoglobin, MCV, MCH and MCHC (P> 0.05). But decreases hematocrit and granulocytes and increases monocytes (P <0.05). The results of biochemical factors showed the amount of glucose and cortisol increased in all treatments and the amount of triglyceride, cholesterol, total protein, albumin, calcium, potassium and sodium decreased in all treatments (P <0.05). Also, the results of steroid hormone analysis showed that by injecting different doses of Ovulin hormone, the amount of testosterone, estradiol and progesterone in all treatments increases (P <0.05). The results of this study showed that doses of 0.6 and 0.7 ml / kg body weight had the best effect on blood indices and steroid hormones studied in female sea carp. However, it is suggested that for maximum efficiency and economic efficiency, a dose of 0.6 ml / kg be used to female sea carp broodstocks.